mouse anti p57 kip2  (Santa Cruz Biotechnology)

Journal: Scientific reports

Article Title: Exploring the significance of interleukin-33/ST2 axis in minimal change disease.

doi: 10.1038/s41598-023-45678-z

Figure Lengend Snippet: Figure 3. Localization of IL-33 and p57 in glomeruli. Representative images of kidney biopsy specimens of control kidney (CON) and MCD tissue stained for IL-33 (red) and p57 (green) and counterstained with DAPI (blue). (A) IL-33 was observed in the nucleus of endothelial cells of glomerular vascular poles, as well as arteries and arterioles (not shown). (B) In patients with MCD, IL-33 was detected in the cytoplasm of glomerular epithelial cells confirmed as positive for the podocyte nuclear marker p57. Original magnification × 400.

Article Snippet: The sections were then washed with PBS and incubated with mouse monoclonal anti-p57 Kip2 antibody (working dilution 1:600; Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4 °C overnight.

Techniques: Control, Staining, Marker

Journal: International journal of cancer

Article Title: Relationship of DNA methylation to mutational changes and transcriptional organization in fusion-positive and fusion-negative rhabdomyosarcoma

doi: 10.1002/ijc.32006

Figure Lengend Snippet: A, Volcano plot displaying differentially methylated probes between PAX3-FOXO1- and PAX7-FOXO1-positive RMS tumors. Red and pink dots denote hypermethylated and hypomethylated probes in PAX3-FOXO1- versus PAX7-FOXO1-positive tumors, respectively. CDKN1C promoter methylation (B) and RNA expression (C) levels in PAX3-FOXO1- and PAX7-FOXO1-positive RMS tumors. D, Correlation plot of DNA methylation versus RNA expression levels for CDKN1C in PAX3-FOXO1- and PAX7-FOXO1-positive RMS tumors. E, Representative CDKN1C immunohistochemical staining results in PAX3-FOXO1- and PAX7-FOXO1-positive tumors. Upper, CDKN1C negative staining in a PAX3-FOXO1-positive tumor; Lower, strong staining in a PAX7-FOXO1-positive tumor. Abbreviations: P3F, PAX3-FOXO1; P7F, PAX7-FOXO1.

Article Snippet: Immunohistochemistry (IHC) was performed using a mouse monoclonal anti-CDKN1C antibody (p57 Kip2 Ab-6, Thermo Scientific).

Techniques: Methylation, RNA Expression, DNA Methylation Assay, Immunohistochemical staining, Staining, Negative Staining

Journal: Nucleic Acids Research

Article Title: FHL2 interacts with and acts as a functional repressor of Id2 in human neuroblastoma cells

doi: 10.1093/nar/gkp332

Figure Lengend Snippet: Downstream targets of E47–Id2 transcriptome are regulated by FHL2 in SK–N–SH neuroblastoma cells. ( A ) SK–N–SH cells were transiently transfected with the FHL2 expression vector or empty vector and cultured for the indicated times. Expression of the indicated transcript abundance was analyzed by qPCR values. HPRT was used as an internal control. All experiments were repeated at least three times, and the results are expressed as mean ± SEM. FHL2, Id2, E47, p57 Kip2 and β-actin protein levels in transfected SK–N–SH cells analyzed by western blot is shown at the bottom. ( B ) SK–N–SH cells were transiently cotransfected with the indicated combinations of plasmids expressing E47, Id2 and FHL2. Cell lysates were analyzed for the expression of p57 Kip2 , IGF2, E47, Id2, FHL2 and β-actin. ( C ) SK–N–SH cells were transiently transfected with the indicated expression vector or different combinations. After 48 h transfection, total RNA was extracted and the abundance of p57 Kip2 was analyzed by qPCR. HPRT was used as an internal control. All experiments were repeated at least three times, and the results are expressed as mean ± SEM (* P < 0.05; N , P > 0.05).

Article Snippet: The rabbit anti-Flag (Sigma), Myc tag (Abcam, Cambridge, MA), GAL4DBD (Clontech), Id2 (Santa Cruz Biotechnology or Abcam), E47 (Santa Cruz), β-actin (Santa Cruz), FHL2 antibodies (Aviva Systems Biology, Beijing) and the mouse anti-p57 Kip2 antibody (Santa Cruz) were used for immunoprecipitation or immunoblotting.

Techniques: Transfection, Expressing, Plasmid Preparation, Cell Culture, Control, Western Blot